Journal: The EMBO Journal

Article Title: SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation

doi: 10.1038/s44318-025-00369-5

Figure Lengend Snippet: Reagents and tools table

Article Snippet: The cells were treated with reagents including the ferroptosis inducers RSL3 (Selleck, S8155) and erastin (Selleck, S7242), ferroptosis inhibitors Ferrostatin-1 (Fer-1) (Selleck, S7243), apoptosis inhibitor Z-VAD-FMK (Z-V) (Selleck, S7023), necrosis inhibitor Necrostatin-1 (Nec-1) (Selleck, S8037), autophagy inhibitor Bafilomycin A1 (Baf-A1) (Selleck, S1413), SLC25A1 inhibitor BTA (Macklin, B801861), ACLY inhibitor SB204990 (Targetmol, T16861), dimethyl Citrate (MCE, HY-N9542), and sodium acetate (Targetmol, T23377).

Techniques: Membrane, Magnetic Beads, Recombinant, Control, Sequencing, Software, Cell Viability Assay, Staining, Purification, SYBR Green Assay, Citrate Assay, Isolation, Clinical Proteomics, GSSG Assay, AST Assay, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Hyperosmotic stress enhances cytotoxicity of SMAC mimetics

doi: 10.1038/cddis.2017.355

Figure Lengend Snippet: SMAC mimetic-induced cell death under hypertonic conditions involves TNF. ( a and b ) L929 cells were grown in medium supplemented with 75 mM NaCl and challenged with the indicated concentrations of ( a ) BV6 and ( b ) LCL161 in the presence and absence of etanercept (400 μ g/ml). ( c ) Cells were grown as above and treated with the indicated concentrations of BV6 in the presence and absence of 75 μ M necrostatin-1 (Nec-1). ( d ) L929 cells were grown in medium supplemented with 75 mM NaCl and challenged with the indicated concentrations of birinapant in the presence and absence of the pan-caspase inhibitor zVAD-fmk (10 μ M) and the RIPK3 inhibitor GSK’872 (2 μ M). Shown are data points and mean±S.E.M. from three independent experiments. Biri, birinapant

Article Snippet: Necrostatin-1 (Nec-1): Stress-Marq (Victoria, Canada); Etanercept was obtained from Pfizer (Berlin, Germany).

Techniques: